Number of observed OTUs detected at week zero (T0) and week eight (T8) in civilian and navy trainee groups. Number of OTUs at T0 and T8 in different categories: cigarette smoking (a), alcohol consumption (b), sleep duration (c), physical exercise (d), and body mass index (BMI) (e). Values are expressed as means ± SEM. Asterisk (*) indicates statistically significant (p < 0.05) differences between groups based on Wilcoxon matched-pairs signed-rank test. At T8, the navy trainees had stopped smoking and drinking alcohol, and had been instructed to do physical exercise for 5 h per day and obey the 8 h sleep schedule.