Figure 3.

Analysis of U87 and U-7-C cells migration by the in vitro wound-healing assay. The phase images of U87 cells when the (a) wound created at 0 h, (b) phase images of wound closure at 24 h, (c) fluorescence images of wound closure at 24 h, the nucleus of the cells are labeled with DAPI and displayed in blue, dead cells are PI-stained and shown in red, yellow lines present the wound area created at 0 h. The same settings were applied for U87-C (d–f). The images acquired with 10× magnification; the scale bar shows 100 µm. (g) The number of migrated cells at 0 and 24 h. The results represent the mean ± standard deviation of two independent experiments. There was no significant difference according to the Student’s unpaired, two-tailed t-test, p = 0.9051.