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. 2020 Sep 11;11(9):845. doi: 10.3390/mi11090845

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Migration of single cells in the microfluidic cell culture chamber. Coordinates of the cells in the microfluidic cell culture device were measured every 12 h between 48 to 120 h. Movement of the U87 cells (a) at x-y axes (b) on the x-axis, (c) on the y-axis. Movement of the U87-C cells (d) at x-y axes, (e) on the x-axis, (f) on the y-axis. The number of analyzed cells for each group is 20. U87 indicates that cells cultured in DMEM medium, U87-C defines that cells were grown in 50% DMEM and 50% macrophage-used RPMI medium. Each color represents the single cells and color coding was consistent in each group.

Migration of single cells in the microfluidic cell culture chamber. Coordinates of the cells in the microfluidic cell culture device were measured every 12 h between 48 to 120 h. Movement of the U87 cells (a) at x-y axes (b) on the x-axis, (c) on the y-axis. Movement of the U87-C cells (d) at x-y axes, (e) on the x-axis, (f) on the y-axis. The number of analyzed cells for each group is 20. U87 indicates that cells cultured in DMEM medium, U87-C defines that cells were grown in 50% DMEM and 50% macrophage-used RPMI medium. Each color represents the single cells and color coding was consistent in each group.