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. 2020 Sep 11;9(9):1187. doi: 10.3390/plants9091187

Figure 2.

Figure 2

Scheme of T-DNA gene constructs for studying the localization of gene products in a plant cell. 1 and 2—constructs for studying the localization of gene products in a plant cell depending on the localization of the signal peptide of the target gene in the N- or C-terminal region of the target protein, respectively. GR or RG—hybrid gene where the target gene (G) is transcriptionally and translationally fused with the gene coding for reporter protein (R) under the control of a strong constitutive promoter (P), Sp—signal peptide for target protein. 3—positive control, a gene construct carrying the specific markers for plant cell compartments under the control of the same promoter; 4—negative control, a gene construct carrying only the gene coding for a reporter protein under the control of the same promoter; P—a strong constitutive promoter; Lb, R—T-DNA left and right borders.