Table 4.
List of primers for PSY1 gene amplification, sequencing, and expression analysis.
| Primer | Sequence (5′→3’) | Application |
|---|---|---|
| PSY1geneF | AGTGGGAATCTACTAGGAGT | Gene amplification and sequencing |
| PSY1geneR | TTATCTTTGAAGAGAGACAGTTT | |
| tPSY_F6 | CTATCTGGGCAATATATGGTG | Gene sequencing |
| tPSY_F7 | TCTCGTCCTAGATACTACAC | |
| tPSY_F8 | CAGTGACGAGCCATGATC | |
| tPSY_F9 | TTGAGCTTGTCGTTCTCAGT | |
| PSY1rtF | CTGAGATCTACCAATGAGTTAG | qRT-PCR, PSY1 |
| PSY1rtR | TCTCGGGAGTCATTAGCATAG | |
| prPSY1-F | GTTGGATTTGCATGTAGACC | Promoter/5′-UTR amplification and sequencing |
| tPSY_F2 | GGCTAAATCGAAAATYGAATC | |
| tPSY_F3 | TAACTTTCTATTGCTTTGCTAGTG | |
| tPSY_F4 | TGGTAGGTAATATTGCTGATTTTG | |
| Actin 2/7-F | CATTGTGCTCAGTGGTGGTTC | qRT-PCR, reference genes |
| Actin 2/7F-R | TCTGCTGGAAGGTGCTAAGTG | |
| Expressed-F | GCTAAGAACGCTGGACCTAATG | |
| Expressed-R | TGGGTGTGCCTTTCTGAATG |