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. 2020 Aug 25;9(9):1095. doi: 10.3390/plants9091095

Figure 3.

Figure 3

Root phenotype analysis of iro3 mutants. (a) Root growth performance of WT and iro3 mutants grown under +Fe or −Fe conditions. (b–e) Root parameters of WT and iro3 mutants grown under +Fe or −Fe conditions. Root biomass (b), root surface area (c), total root length (d), and total root tip number (e) of WT and iro3 mutants under +Fe and −Fe conditions. Fourteen-day-old seedlings were transferred to −Fe or +Fe for 8 d. The iro3-1 mutant contains an insertion of ‘A’; the iro3-2 mutant contains an insertion of ‘T’. Data are given as the means ± SD of six biological replicates. All data were compared with the WT. Asterisks indicate significant differences of WT and iro3 mutants based on two-tailed Student’s t test (* p < 0.05, ** p < 0.01).