Figure 6.

Biofunctionalization inside the 3D printed PMMA channel. (a) Fluorescently labelled biomolecules can be covalently bound to surfaces by photobleaching the fluorophore upon exposure with light patterns. (b) 2 mm wide embedded PMMA channel printed on a PMMA substrate with biofunctionalization inside the printed channel indicated by dotted lines (scale bar: 10 mm). (c) The bottom surface of the 3D printed channel on top of the commercial PMMA slide was functionalized with different biotin patterns by photobleaching of F5B. Patterns were illuminated for 3 min (patterns 1 and 2) and 5 min (patterns 3 and 4). The patterns were visualized via staining with STV-Cy3 (scale bar: 750 µm). Signal-to-noise ratios were calculated for all four images and were on average 29 for 3 min illumination and 11 for 5 min. (d) Close-up of patterns which were illuminated for 3 min.