Figure 1.

Neutrophils rapidly emigrated from bone marrow in response to infection with vesicular stomatitis virus (VSV). Six- to eight-week-old female C57BL/6 mice were injected intravenously with 1 × 10⁹ plaque-forming units of VSV or phosphate-buffed saline (PBS) and euthanized three- or 24-hours (h) later. Bone marrow flushed from femurs was used to quantify neutrophils by flow cytometry. Representative dot plots and graphs with means and standard deviations (SD) (n = 8/group) are shown for percentages of (A) CD45⁺ leukocytes that were neutrophils (defined as CD45⁺Ly6G^hiCD11b^hi), (B) neutrophils that were mature (defined by the additional expression of CD101), and (C,D) mature neutrophils expressing the lung-homing chemokine receptors (C) CXCR2 or (D) CXCR4. Data were assessed by one-way analysis of variance with Tukey’s multiple comparisons test. (ns = not significantly different).