Table 1.
Candidates in the flavonoid biosynthesis of Croton tiglium. “TRINITY” prefix of all sequence names was omitted for brevity. Candidates are sorted by their position in the respective pathway and decreasing similarity to bait sequences. Transcripts per million (TPM) values of the candidates in different tissues are shown: leaf (SRR6239848), stem (SRR6239849), inflorescence (SRR6239850), root (SRR6239851), and seed (SRR6239852). Displayed values were rounded to the closest integer, and thus extremely low abundances appear as 0. A full table with all available RNA-Seq samples and transcript abundance values for all candidates is available in the Supplementary Information (Supplementary S6).
| Sequence ID | Function | Leaf | Stem | Inflorescence | Root | Seed |
|---|---|---|---|---|---|---|
| DN23351_c0_g1_i2 | CtPALa | 18 | 10 | 0 | 1 | 29 |
| DN32981_c5_g1_i1 | CtPALb | 0 | 0 | 0 | 0 | 0 |
| DN32981_c5_g1_i16 | CtPALc | 4 | 3 | 0 | 0 | 0 |
| DN32981_c5_g1_i9 | CtPALd | 0 | 0 | 0 | 0 | 0 |
| DN32981_c5_g1_i17 | CtPALe | 0 | 25 | 0 | 5 | 10 |
| DN32981_c5_g1_i12 | CtPALf | 5 | 233 | 0 | 18 | 48 |
| DN32981_c5_g1_i5 | CtPALg | 0 | 318 | 0 | 3 | 3 |
| DN32981_c5_g1_i13 | CtPALh | 0 | 3 | 0 | 0 | 0 |
| DN32981_c5_g1_i14 | CtPALi | 0 | 0 | 0 | 0 | 0 |
| DN23351_c0_g2_i1 | CtPALj | 18 | 1 | 0 | 9 | 12 |
| DN32464_c6_g3_i2 | CtC4Ha | 122 | 110 | 2 | 77 | 233 |
| DN15593_c0_g1_i1 | CtC4Hb | 0 | 0 | 0 | 0 | 3 |
| DN32164_c5_g1_i2 | Ct4CLa | 46 | 19 | 1 | 2 | 113 |
| DN50385_c0_g1_i1 | CtCHSa | 3 | 6 | 0 | 1 | 588 |
| DN27125_c0_g1_i1 | CtCHI Ia | 11 | 2 | 19 | 3 | 88 |
| DN33424_c3_g3_i1 | CtF3Ha | 4 | 21 | 1 | 2 | 342 |
| DN33407_c7_g7_i2 1 | CtFNS IIa | 1 | 7 | 0 | 95 | 1 |
| DN33407_c7_g7_i1 1 | CtFNS IIb | 0 | 3 | 1 | 4 | 2 |
| DN27999_c0_g1_i2 1 | CtFNS IIc | 0 | 2 | 0 | 75 | 0 |
| DN33407_c7_g6_i4 1 | CtFNS IId | 0 | 0 | 0 | 22 | 0 |
| DN252_c0_g1_i1 | CtF3′Ha | 111 | 62 | 0 | 9 | 165 |
| DN32466_c16_g7_i1 | CtF3′5′Ha | 0 | 0 | 0 | 7 | 266 |
| DN32466_c16_g7_i3 | CtF3′5′Hb | 0 | 0 | 0 | 0 | 3 |
| DN25915_c0_g1_i3 | CtFLSa | 18 | 19 | 0 | 0 | 84 |
| DN25915_c0_g2_i1 | CtFLSb | 0 | 1 | 0 | 1 | 2 |
| DN27402_c0_g1_i3 | CtDFRa | 0 | 0 | 0 | 0 | 51 |
| DN32893_c8_g1_i1 | CtANSa | 0 | 0 | 0 | 0 | 25 |
| DN33042_c3_g1_i3 | CtLARa | 3 | 2 | 0 | 1 | 101 |
| DN30161_c9_g1_i2 | CtANRa | 0 | 1 | 0 | 1 | 375 |
| DN30161_c9_g1_i3 | CtANRb | 0 | 0 | 0 | 0 | 3 |
1 These sequences might encode non-functional enzymes or enzymes with a different function (see results and discussion for details) but represent the best flavone synthase (FNS) II candidates. The background color shows the transcript abundance.