Dot plot of glutarylcarnitine (C5DC) proficiency test raw and harmonized data across US public health newborn screening (NBS) labs from 2019. Dot plots of raw (A,B) harmonized glutarylcarnitine (C5DC) proficiency test (PT) data reported by US public health NBS laboratories in 2019. Laboratory names are de-identified. Laboratory PT values are grouped by analytical method, and these unique methods are denoted by color and number. Each “•” indicates a unique US public health NBS laboratory’s PT concentration for C5DC. The black solid line represents the mean of PT values in each plot. Method 1: Derivatized—MS/MS LDT; Method 2: PerkinElmer NeoGram AAAC Tandem Mass Spectrometry kit (Waltham, MA, USA); Method 3: Perkin Elmer NeobaseTM 2 Non-derivatized MSMS kit (Waltham, MA, USA); Method 4: Non-derivatized—MS/MS LDT; Method 5: Perkin Elmer NeobaseTM Non-derivatized MSMS kit (Waltham, MA, USA).