Figure 1.

Effect of antibodies on enzymatic cyclase activity. Soluble (S) and solubilized membrane (PS) fractions of barley plastids were combined to obtain cyclase activity (red). Addition of antibodies raised against ferredoxin-NADPH oxidoreductase (FNR) (a, green) or Fd (b, green) significantly inhibited activity. Assays with addition of antibodies raised against barley brassinosteroid receptor BRI1 were used as controls to ensure that there was no general inhibitory effect of antibodies. Three parallel assays were performed, and the displayed curves are averages of the three replicates. ***, p < 0.001.