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. 2020 Sep 8;9(9):1157. doi: 10.3390/plants9091157

Figure 4.

Figure 4

Enzymatic activity of recombinant XanL in combination with spinach Fd (ferredoxin) and spinach FNR (ferredoxin-NADPH oxidoreductase). Used concentrations of Fd and FNR were 0.5 µg/μL and 0.75 milli-units/μL, respectively. (a) Cyclase activity assays with recombinant XanL co-expressed with Ycf54 (XanL[coYcf54]). Product formation increased linearly with the amount of added XanL[coYcf54] (y = 6.99x + 12.7, R2 = 0.983, p = 0.0077). No activity was obtained when Fd or FNR was omitted from the assay. (b) Assays performed with recombinant XanL expressed without Ycf54. No activity was detected. (c) Assays performed with recombinant XanL (83 μg) and Ycf54 expressed separately. No activity was detected.