Table 1.

List of oligonucleotides used.

Name	Sequence	Description
Duet_XanL_TEV_Forward	5′-TGAGAATCTTTATTTTCAGGGCAA GCCGGGTAGCCCGAAGAAACGTGGCA	Forward primer to add TEV site to pETDuet-1HvXanL constructs
Duet_XanL_TEV_Reverse	5′-CCCTGAAAATAAAGATTCTCAAG CTTGTCGACCTGCAGGCGCGCCGAGCT	Reverse primer to add TEV site to pETDuet-1HvXanL constructs
DuetTEV2x His_Forward	5′-CACCACCATCATAGCCAGGATCC GAATTCGAGCTCGGCGCGCCTGCAGGT	Forward primer to add 2 extra Histidine residues to make a 8x His tag
DuetTEV2x His_Reverse	5′-GGATCCTGGCTATGATGGTGGTG ATGATGGTGATGGCTGCTGCCCATGGT	Reverse primer to add 2 extra Histidine residues to make a 8x His tag
DuetTEV4x His_Reverse	5′-GGATCCTGGCTATGATGGTGGTGATG ATGGTGATGATGATGGCTGCTGCCCATGGT	Reverse primer to add 2 extra Histidine residues to make a 10x His Tag. This primer was run with DuetTEV2xHis_Forward