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Extracted ion chromatogram of glycosyl inositol phospho ceramides (GIPCs) in salad samples analyzed by RP-HRMS/MS analysis using ddMS2 in positive (red) and negative (blue) ion modes. Assigned GIPCs belong to the Hex-HexA-IPC series with a t18:1 long-chain base (LCB) and varying chain length of the hydroxylated saturated fatty acids. Retention times coincided in positive and negative ion modes. Increasing carbon numbers result in belated elution.
