Fig. 1.

Frequency of CD4+ (A and B) and CD8+ (C and D) SIV-specific cytokine secreting T cells following DNA immunization. Mice were immunized twice, 4 weeks apart with SIV Gag and Env DNA (30μg) by IM or EP +/− pIL-12 (0.3μg). Splenocytes were harvested two weeks after the second immunization, incubated ex vivo for 5h with Gag pooled peptides, the dominant Gag peptides: DD13 (CD4) and AL11 (CD8) (A and C) or Env pooled peptides (B and D), anti-CD28 and brefeldin A before being analyzed by flow cytometry for production of IFNγ, IL-2 and TNFα. The total frequency of cytokine secreting T cells (%) producing IFNγ, IL-2 and TNFα are shown and bars represent the median, n = 4 and data are representative of 2 experiments. *p < 0.05, significantly different from animals immunized by IM.