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. Author manuscript; available in PMC: 2020 Oct 19.
Published in final edited form as: Vaccine. 2013 Aug 14;31(42):4749–4758. doi: 10.1016/j.vaccine.2013.08.011

Fig. 5.

Fig. 5.

Effect of high dose pIL-12 on the frequency (A) and quality (B/C) of memory CD4 and CD8 (D) SIV-specific cytokine secreting T cells. Mice were immunized twice, 4 weeks apart with SIV Gag and Env DNA (30μg) by EP with low dose (0.3μg) or high dose (25μg) pIL-12 before a boost with rAd5 vectors expressing Gag and Env. Splenocytes were harvested at week 14, incubated ex vivo for 5h with Gag pooled peptides, the dominant DD13/AL11 peptides or Env pooled peptides, anti-CD28 and brefeldin A before being analyzed by flow cytometry for total production of IFNγ, IL-2 and TNFα (A/D) or IFNγ, IL-2 and TNFα in any combination (B/C) with bars representing the median, n = 4 and data are representative of 2 experiments, *p < 0.05, significantly different from animals immunized by EP.