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. 2020 Sep 8;25(18):4106. doi: 10.3390/molecules25184106

Figure 2.

Figure 2

Detection of unreacted azido groups in AzidoSilk film on cell culture plates after SPAAC reactions with DBCO-mPEG of different chain lengths: (A) 5 kDa, (B) 10 kDa, (C) 20 kDa, and (D) 30 kDa. The concentrations of DBCO-mPEGs were changed from 30 to 300 μm to find an appropriate reaction condition under which to achieve saturation of reactions with DBCO-mPEG. Unreacted azido groups were reacted with sulfo-DBCO-biotin. Biotins were detected by coloration with an HRP substrate after conjugation with HRP-streptavidin. The averaged UV absorbance at 450 nm from four wells was plotted with error bars of standard deviation.