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. 2020 Sep 8;25(18):4106. doi: 10.3390/molecules25184106

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Number of adherent NIH3T3 fibroblasts on the surfaces of normal silk fibroin and AzidoSilk films when treated with DBCO-mPEG of different chain lengths. Fibroblasts were cultured for 2 h at 37 °C in Eagle MEM (minimum essential media) without FBS (fetal bovine serum). Cells were counted by the lactate dehydrogenase (LDH) method and the results were averaged. The error bars represent standard deviation (n = 8 for TCPS and n = 4 for the others). Statistical significance by the Tukey–Kramer test is shown. N.S. denotes no statistical significance (p > 0.05).