Table 1.
Deduced functions of ORFs in the dnacin B1 biosynthetic gene cluster.
| ORF | Number of Amino Acids |
Proposed Function | Sequence Similarity (Protein, Origin) | % Identity/ Similarity |
Accession No. |
|---|---|---|---|---|---|
| DinR1 | 951 | ABC transporter | IQ63_41860, Streptomyces acidiscabies | 64/75 | KND24720.1 |
| ORF1 | 117 | unknown | SD37_10880, Amycolatopsis orientalis | 55/75 | ANN21703.1 |
| ORF2 | 175 | protease inhibitor | AC230_04780, Streptomyces caatingaensis | 38/54 | KNB54254.1 |
| ORF3 | 395 | histidine kinase | A9W97_15640, Mycobacterium gordonae | 51/60 | OBJ88517.1 |
| ORF4 | 159 | ATP-binding protein | RKT69099.1, Saccharothrix variisporea | 45/51 | DFJ66_2293 |
| ORF5 | 306 | epimerase | DI639_00155, Leifsonia xyli | 74/83 | PZO61411.1 |
| DinR2 | 228 | TetR family transcriptional regulator | STRAU_2618, Streptomyces aurantiacus | 58/71 | EPH44178.1 |
| DinA | 534 | flavin adenine dinucleotide (FAD)-linked oxidase | NapU, Streptomyces lusitanus | 64/75 | AGD80628.1 |
| DinB | 471 | gamma-aminobutyraldehyde dehydrogenase | MCBG_02966, Micromonospora sp. M42 | 53/65 | EWM65833.1 |
| DinC | 741 | peptidase | NapG, Streptomyces lusitanus | 48/56 | AGD80614.1 |
| DinD | 183 | flavin mononucleotide (FMN) reductase | SsuE, Streptomyces noursei | 58/67 | WP_067345371.1 |
| DinR3 | 707 | regulatory protein | NapR3, Streptomyces lusitanus | 55/68 | AGD80615.1 |
| DinE | 103 | 4-amino-4-deoxychorismate mutase | PapB, Streptomyces venezuelae | 34/53 | BAD21142.1 |
| DinF | 307 | 4-amino-4-deoxyprephenate dehydrogenase | PapC, Streptomyces venezuelae | 40/51 | BAD21141.1 |
| DinG | 300 | unknown | CLV43_102760, Umezawaea tangerina | 47/57 | PRY45195.1 |
| DinH | 796 | adenylation domain | NapH, Streptomyces lusitanus | 51/60 | AGD80616.1 |
| DinI | 342 | non-heme iron hydroxylase | NapI, Streptomyces lusitanus | 63/75 | AGD80617.1 |
| DinJ | 1482 | NRPS | NapJ, Streptomyces lusitanus | 68/79 | AGD80618.1 |
| Module 5 | C-A-PCP-RE | ||||
| DinK | 66 | MbtH-like protein | NapK, Streptomyces lusitanus | 73/84 | AGD80619.1 |
| DinL | 1125 | NRPS | NapL, Streptomyces lusitanus | 54/64 | AGD80620.1 |
| Module 4 | C-A-PCP | ||||
| DinM | 252 | thioesterase II | NapM, Streptomyces lusitanus | 51/64 | AGD80621.1 |
| DinN | 632 | AMP-dependent synthetase and ligase | NapN, Streptomyces lusitanus | 64/74 | AGD80622.1 |
| Loading | AL-PCP | ||||
| DinO | 3049 | NRPS | NapO, Streptomyces lusitanus | 52/63 | AGD80623.1 |
| Module 1 | C-A-PCP | ||||
| Module 2 | C-A-PCP | ||||
| Module 3 | C-A-PCP | ||||
| DinP | 223 | hypothetical protein | NapP, Streptomyces lusitanus | 45/59 | AGD80633.1 |
| DinR4 | 909 | ABC transporter | B0I31_10111, Saccharothrix carnea | 56/67 | PSL57800.1 |
| DinR5 | 280 | ABC transporter | CLV69_102810, Yuhushiella deserti | 53/69 | TDX97704.1 |
| DinQ | 336 | 3-oxoacyl-synthase III (KS) | NapE, Streptomyces lusitanus | 72/80 | AGD80612.1 |
| DinS | 754 | Transketolase | NapD, Streptomyces lusitanus | 59/68 | AGD80611.1 |
| DinT | 76 | acyl carrier protein (ACP) | NapC, Streptomyces lusitanus | 63/75 | AGD80633.1 |
| DinU | 306 | transketolase | NapB, Streptomyces lusitanus | 68/77 | AGD80609.1 |
| DinV | 706 | 4-amino-4-deoxychorismate synthase | PapA, Streptomyces venezuelae | 48/58 | BAD21140.1 |
| DinW | 486 | 4-hydroxyphenylacetate 3-monooxygenase | EWI31_24745, Streptomyces tsukubensis | 56/69 | TAI42134.1 |
| DinR6 | 613 | ABC transporter | CLV43_102777, Umezawaea tangerina | 70/78 | PRY45212.1 |
| DinR7 | 601 | ABC transporter | DIU55_13605, Firmicutes bacterium | 51/66 | PZN68908.1 |
| DinX | 477 | monooxygenase | NapA, Streptomyces lusitanus | 69/76 | AGD80608.1 |
| DinR8 | 818 | UV-repair protein | NapR1, Streptomyces lusitanus | 75/84 | AGD80606.1 |
| DinY | 244 | methyltransferase | QncJ, Streptomyces melanovinaceus | 47/65 | AGD95052.1 |
| DinZ | 118 | long-chain-fatty-acid--CoA ligase | NCTC13184_06982, Nocardia africana | 60/81 | SUA48430.1 |
| ORF6 | 182 | sugar O-acetyltransferase | E1091_12580, Micromonospora fluostatini | 64/72 | TDB92781.1 |
| DinR9 | 304 | LysR family transcriptional regulator | AFR_16085, Actinoplanes friuliensis | 57/69 | AGZ41499.1 |
| ORF7 | 104 | transposase | CLV43_10550, Umezawaea tangerina | 56/61 | PRY41292.1 |