Table 3.
The unsaturated fatty acid (UFA) compounds corresponding to the six principal peaks in the HPLC chromatogram of Rana chensinensis ovum oil (RCOO).
| Peak | RT in MS a (min) | [M − H]− | Mass Error (mDa) | Molecular Formula | Proposed Compound | RT in HPLC b (min) |
|---|---|---|---|---|---|---|
| 1 | 1.287 | 301.1901 | 2.0 | C20H30O2 | EPA | 10.29 |
| 2 | 1.400 | 277.1917 | 2.3 | C18H30O2 | ALA | 11.26 |
| 3 | 1.467 | 327.2035 | 1.9 | C22H32O2 | DHA | 12.19 |
| 4 | 1.614 | 303.2077 | 2.5 | C20H32O2 | ARA | 13.91 |
| 5 | 1.806 | 279.2090 | 2.8 | C18H32O2 | LA | 15.66 |
| 6 | 2.427 | 281.2235 | 2.1 | C18H34O2 | OA | 22.53 |
a The retention time in the total ion current (TIC) chromatogram. b The retention time in the HPLC chromatogram. EPA: eicosapentaenoic acid; ALA: α-linolenic acid; DHA: docosahexaenoic acid; ARA: arachidonic acid; LA: linoleic acid; OA: oleic acid.