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Fluorescence lifetimes for FABT measured with the TCSPC method after excitation with a 294 nm diode.
| Buffer | Constituent | Lifetime [ns] | Fraction [%] |
|---|---|---|---|
| EtOH neutral | τ1 | 1.59 ± 0.054 | (13.9 ± 1.2) |
| τ2 | 0.794 ± 0.003 | (86.1 ± 1.2) | |
| EtOH basic | τ1 | 1.79 ± 0.01 | (31.1 ± 0.4) |
| τ2 | 0.22 ± 0.01 | (68.9 ± 0.4) | |
| EtOH acidic | τ1 | 5.0 ± 0.4 | (1.8 ± 0.1) |
| τ2 | 0.73 ± 0.1 | (98.2 ± 0.1) | |
| Chloroform | τ1 | 27.3 ± 0.2 | (34.7 ± 0.1) |
| τ2 | 1.67 ± 0.02 | (25.4 ± 0.4) | |
| τ3 | 0.22 ± 0.01 | (39.9 ± 0.3) |
