Figure 4.

Effects of DNE on IgE-mediated passive cutaneous anaphylaxis. (A) The ear skin of mice (n = 6/group) was sensitized by intradermal injection of anti-DNP IgE (0.5 mg/site) for 48 h. DNE (100 and 200 mg/kg) or dexamethasone (10 mg/kg) was orally administered before the intravenous (i.v.) injection of a DNP-BSA and 4% Evans blue (1:1) mixture. After 30 min, the thickness of both ears was measured. (B) Ear thickness was measured with a dial thickness gauge. (C) The dye was extracted and quantified using a spectrophotometer. Data represent the mean ± SEM from three independent experiments. *** p < 0.001, Tukey’s test, significantly different from Anti-DNP IgE plus DNP-BSA with PBS-treated group. Anti-DNP IgE, anti-dinitrophenyl immunoglobulin E; DNP-BSA, dinitrophenyl-Bovine serum albumin; BNE, Boehmeria nivea extract; Dexa, dexamethasone.