Figure 3.

(A) Effect on K562 cell adhesion on fibronectin coated plates. Cells were pre-incubated with 50 µM RGDechi15D or RGDechi (used as positive control) or 10 μg/mL anti-αvβ5 antibody, for 30 min at 4 °C and then seeded on fibronectin coated plates at 37 °C. Cell adhesion was evaluated after 1 h of incubation using crystal violet reagent. (B) Effect on K562 adhesion on α5β1 antibody coated plates. K562 were pre-incubated with peptides (50 µM) for 30 min at 4 °C then seeded on anti-α5β1 coated plates. The results are presented as the percentage of adherent cells respect to the control (untreated cells) and are expressed as means ± SE of at least three independent experiments performed in triplicate. Statistical significance was analyzed using Student’s t test, unpaired, two-sided (* p < 0.05).