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. 2020 Sep 19;25(18):4298. doi: 10.3390/molecules25184298

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Angiogenesis Assay. The in vitro angiogenesis assay, using extracellular matrix (ECM) was performed to characterize the anti-angiogenic effect mediated by treatment with the RGDechi15D peptide. (A) Representative phase-contrast micrographs of tubular structures in cultured Human umbilical vein endothelial cells (HUVEC) previously exposed to 50 μM RGDechi15D peptide. Magnification: 10×. (B) The bar graph illustrates the significant decrease in the percentage of branch points after RGDechi15D treatment compared to control cells. Data are shown as mean (± SE) of two independent experiments performed in duplicate (* p < 0.05).