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. 2020 Sep 19;25(18):4298. doi: 10.3390/molecules25184298

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Western Blotting of pAKT. Analysis of the AKT phosphorylation state after peptide treatment was carried out on HepG2. Cells were treated with the RGDechi15D peptide for 1 h. (A) Total cellular extracts (50 μg) were resolved by SDS PAGE and analyzed by Western blotting with anti-pAKT (Ser473) and anti-AKT antibodies. The Western blotting is representative of three independent experiments. (B) Bar graph illustrates the percentage of band quantification respect to the control. 1 = Control, 2 = RGDechi15D, 3 = Scrambled peptide. The results are expressed as mean ± SE of percentage of pAKT normalized to the density of AKT.