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. 2020 Sep 5;25(18):4058. doi: 10.3390/molecules25184058

Figure 1.

Figure 1

Inhibitory effect of retrofractamide C (RAC) on lipopolysaccharide (LPS)-induced nitric oxide (NO) and PGE2 production and iNOS and COX2 protein expression in J774A.1 cells. (A) Chemical structure of retrofractamide C (RAC). (B) Cytotoxicity of RAC. J774A.1 cells were treated with the indicated concentrations of dexamethasone and RAC for 24 h. Cytotoxicity was determined by MTT assay. (C,D) Inhibition of NO and PGE2 production by RAC. J774A.1 cells were pretreated with 10 μM dexamethasone or 1, 3 or 10 μM RAC for 1 h before treatment with 200 ng/mL LPS for 18 h. (C) The secretion of NO was measured by an NO assay. (D) PGE2 production was determined by ELISA. (E) iNOS and COX2 protein expression decreased by RAC treatment. J774A.1 cells were treated with 200 ng/mL LPS for 18 h after treatment with dexamethasone and RAC for 1 h. The iNOS and COX2 protein expression levels were determined by immunoblot assay. The band optical densities were calculated by ImageJ software. Representative data are presented. Values are presented as the mean ± SD of three individual experiments. * p < 0.05, ** p < 0.01 compared with the LPS only-treated group. DEX, 10 μM dexamethasone.