Figure 3.

Effect of RAC on LPS-induced MAPKs and the NF-κB signaling pathway. J774A.1 cells were pretreated with 10 μM dexamethasone or 1, 3 or 10 μM RAC for 1 h before treatment with 200 ng/mL LPS for 30 min to 1 h. (A) The protein expression of MAPKs was determined by immunoblot. Band optical densities were calculated by ImageJ software. (B) NF-κB p65 subunit expression was measured by immunoblot analysis, and the band optical density was calculated by ImageJ software. The results are representative of three independent experiments. Values are presented as the mean ± SD of three individual experiments. * p < 0.05, ** p < 0.01 compared with the LPS only-treated group.