Table 1.

Effect of ST2825 on rhIL-1β-stimulated PBMC.

	Pro-Inflammatory Cytokine Profile
	RPMI	rhIL-1β	* p-Value	rhIL-1β + ST2825	** p-Value
TNF-α	825.3 (304.3–2020)	1158 (648–1918)	ns	409 (289.5–303.8)	ns
IFN-γ	70.3 (18.6–126.6)	167.8 (137.4–184)	ns	26.52 (24.20–33.3)	<0.01
IL-6	434 (171.4–1333)	894.2 (720.4–1795)	ns	982.3 (713.3–2371)	ns
IL-12	1.4 (0.4–1.4)	4.8 (4.4–5.1)	<0.01	4.7 (3.2–5.1)	ns
IL-17A	12.2 (6.3–23.7)	86.8 (74–88.4)	<0.05	81.1 (71–86.2)	ns
G-CSF	131.8 (82.1–243.6)	293.6 (241.9–534.8)	<0.05	402.2 (277.1–573.2)	ns
GM-CSF	1.4 (0.8–2.6)	5.4 (4.9–5.8)	<0.05	5.2 (4–5.7)	ns
IL-2	18.1 (8.1–38.2)	41.1 (37.3–49.6)	ns	28.5 (24.8–29.9)	ns
VEGF	0 (0–88.5)	723.4 (586.2–805.3)	<0.05	843.2 (585.1–954.2)	ns
IL-15	1.2 (0–17.4)	74.1 (69.7–77.3)	<0.05	78.06 (70.65–79.3)	ns
IL-7	14.2 (7.7–17.7)	28.7 (22.1–30.7)	ns	25.7 (16.9–30.1)	ns
	Anti-Inflammatory Cytokine Profile
	RPMI	rhIL-1β	* p-Value	rhIL-1β + ST2825	** p-Value
IL-1Ra	2211 (1165–3543)	3339 (2399–3710)	ns	670 (426–1638)	<0.001
IL-4	3 (1.6–5.6)	13.7 (12.8–14.6)	<0.01	13.5 (12.3–14)	ns
IL-5	17.9 (8.6–40.5)	53 (49.1–60)	ns	45.8 (37.4–57.3)	ns
IL-13	0.6 (0.2–0.8)	1.1 (0.9–1.2)	ns	1 (0.9–1.1)	ns
IL-10	13.3 (5.6–50.5)	43 (27.1–83.6)	ns	22.8 (13.2–35.6)	ns
IL-9	7.9 (5.3–10.5)	14 (12.3–15.3)	<0.01	12.7 (10–16.3)	ns

Data provided in medians and interquartile ranges (25th and 75th). The concentration of cytokines is provided in pg/mL. * p-value determined by Dunn’s test (RPMI vs rhIL-1β). ** p-value determined by Dunn’s test (rhIL-1β vs. rhIL-1β plus ST2825). Not significant (ns). Abbreviations: IL, interleukin; TNF-α, tumor necrosis factor alpha; IFN-γ, interferon gamma; G-CSF, granulocyte colony-stimulating factor; GM-CSF, granulocyte-monocyte colony-stimulating factor; VEGF, vascular endothelial growth factor.