Figure 1.

Antiviral activity of harringtonine against Mosquito-borne Zika virus (ZIKV). The cytotoxicity of harringtonine-treated Vero cells for 48 h was determined by Cell Counting Kit 8 (CCK-8) assays. The viability was determined with a Cell Counting Kit 8 assay. The experiments were carried out in triplicates, and the error bars represent standard deviation (A). Vero cells were infected with ZIKV and were treated with different concentrations of harringtonine for 48 h. The anti-ZIKV ability of harringtonine was analyzed by measuring viral RNA levels (B). The cell supernatants were harvested, and virus titers were assessed by fluorecent focus assay (C). The intracellular protein expression of Env was assessed by western blot (D). The severity of ZIKV infection was determined by IFA (E) and quantification of Vero cells (F). Statistical significance was analyzed from t-test compared with the ZIKV group: * p < 0.05; ** p < 0.01; *** p < 0.001. Scale bar: 50 μm.