Figure 7.

(a,b) Protein expression and molecular mechanisms of EA inhibitory actions in SKBR3 cell line. This plant extract induces an overexpression of E-cadherin, β-catenin, and downregulation of vimentin and fascin, while upregulating pro-apoptotic markers (Bax and Caspase-3), in comparison with their control and inhibiting anti-apoptotic markers (Bcl-2). Furthermore, EA plant extract inhibits the phosphorylation of ErbB2 and β-catenin, as well as the expression of JNK1/2/3. β-actin was used as a control for the proteins amount in this assay. Cells were treated with 100 and 200 μL/mL of EA extract for 48 h, as explained in the materials and methods and the results sections. (a) Blot image and (b) quantification of bands.