Table 2.

Accuracy and precision results for OTN determination by the proposed LC-MS/MS method.

Accuracy and Precision (n = 5, Three days)		LLOQ (25 nM)	MQC (500 nM)	HQC (800 nM)
1st day Intraday	Bias (mean, n = 5)	15.41	−6.30	−1.09
	Average Percent Recovery	115.41%	93.70%	98.91%
	S.D.	14.55	2.01	3.57
	% R.S.D	12.61	2.14	3.64
2nd day Intraday	Bias (mean, n = 5)	10.34	1.49	−0.10
	Average Percent Recovery	110.34%	101.49%	99.90%
	S.D.	15.37	1.90	1.82
	% R.S.D	13.92	1.87	1.82
3rd day Intraday	Bias (mean, n = 5)	13.80	0.98	−0.91
	Average Percent Recovery	113.80%	100.98%	99.09%
	S.D.	9.57	1.83	2.62
	% R.S.D	8.41	1.81	2.64
Interday	Bias (mean, n = 15)	13.40	−1.30	−1.01
	Average Percent Recovery	113.40%	98.70%	98.99%
	S.D.	13.41	4.02	2.89
	% R.S.D	11.83	4.07	2.92