The dashed line of 20 units indicates the cut-off value for anti-Ro52 autoantibody positivity. Out of 371 JIIM patients, 53 (14%) were positive for anti-Ro52 autoantibodies by ELISA. Of these patients, 42 had JDM, 3 had JPM, and 8 had JCTM. Out of 90 juvenile healthy controls, one patient (1.1%) was positive for anti-Ro52 autoantibodies by ELISA.