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. 2020 Sep 9;25(18):4112. doi: 10.3390/molecules25184112

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The influence of EP on mTOR activity. (A) The proportion of Treg (CD4⁺CD25^highFoxP3⁺) and (B) the number of ROS-producing Treg (CD4⁺CD25^high) 72 h after the treatment with EP and/or rapamycin (Rapa). (C) The expression of phosphorylated S6K (pS6K) and (D) phosphorylated Akt (pAkt) normalized to the expression of total protein forms measured by immunoblot 72 h after EP and/or rapamycin administration. Representative blots are shown below the graph. * p < 0.05 represents the significant difference of EP- or rapamycin-treated cells in comparison to untreated control cells.