Figure 1.

Effect of hemin, SFN, QUE and 3,4HPAA in cell viability. (A) RKO cells and (B) CCD841 cells were incubated with increasing concentrations of hemin (0.05–50 μM), SFN, QUE or 3,4HPAA (0.05–200 μM). After 72 h, the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) reduction was detected by absorbance (MTS assay). The results were expressed as percentage of inhibition in relation to the positive control (30 µM puromycin) and calculated as: ((OD_{0.4% DMSO} − OD_sample) × 100)/(OD_{0.4% DMSO} − OD_puromycin). Values are expressed as mean ± SEM, from three independent culture preparations. 3,4HPAA, 3,4-dihydroxyphenylacetic acid; QUE, quercetin; SFN, sulforaphane.