Antimicrobial Activity of Gemini Surfactants with Azapolymethylene Spacer

Abstract

A series of 21 azapolymethylene gemini surfactants were obtained. The synthesis of the title surfactants in one- or two-step reaction proceeds with good yields. The structure and the purity of the synthesized compounds were determined by ¹H and ¹³C NMR, ESI-MS spectra, and elemental analysis. Moreover, 2D COSY, HMBC, and HSQC spectra were performed. The minimal inhibitory concentrations (MIC) of the synthesized compounds were determined against fungi: Candida albicans, Aspergillus niger, Penicillium chrysogenum and bacteria: Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Bacillus subtilis. Also, the critical micelle concentrations (CMC) were determined. The relationship between antimicrobial and surface activity and surfactant structure has been determined.

1. Introduction

Quaternary alkylammonium salts are amphiphilic compounds which consist of hydrophobic and hydrophilic parts. The hydrophilic part constitutes a quaternary nitrogen atom whereas the hydrophobic part is the alkyl chains of different lengths. Two quaternary ammonium compounds are linked by a spacer at polar heads or close to them form gemini surfactants [1,2,3,4,5]. The surface tension and critical micelle concentration of gemini surfactants, as well as their antimicrobial activity, expressed by minimal inhibitory concentration, are significantly lower, even hundreds of times, in comparison to their monomeric analogs [6,7,8,9,10,11,12,13]. This is of great ecological importance because to achieve a similar surface or antimicrobial effect, much smaller amounts of the substance can be used.

In dilute solutions, surfactants exist as single molecules, monomers [1]. As the concentration increases, the surfactants associate to form micelles. The solution passes from the real solution to the colloidal one [14,15,16]. The concentration of the surfactant, in which the physical properties of the solution change rapidly due to the formation of associates, is called the critical micelle concentration (CMC). In concentrated solutions the surfactant above CMC is thermodynamically stable, remaining in the equilibrium with monomers [10,13,17,18].

The hydrocarbon chains, in an aqueous environment, form the core of the micelle while the hydrophilic groups are located outside of the micelle [19]. Therefore, the surfactant structure plays a key role in the formation of micelles [20,21].

Besides the excellent surface activity, gemini surfactants act also as a very efficient microbiocide and corrosion inhibitors, which is directly related to the adsorption capacity of cationic salts on negatively charged surfaces [22,23,24,25,26,27,28,29,30,31]. Adsorption of alkylammonium salt on the cell wall of the microorganism causes damage to the wall, and leakage of potassium ions and low molecular components of the bacterial cell, which in turn leads to cell death [32,33,34,35].

Due to the constantly growing demand for gemini surfactants as microbiocides, we focused on the synthesis of dimeric alkylammonium salts with azapolymethylene spacers and determination of the relationship between the structure of surfactants and their antimicrobial activity against bacteria and fungi. In this study, the critical micelle concentration of obtained compounds was also investigated.

2. Results and Discussion

2.1. Synthesis and Spectroscopic Characterisation of Gemini Surfactants

The 3-methylaza-1,5-pentane-bis(N,N-dimethyl-N-alkylammonium) diiodides (compounds 1–6) and dibromides (compounds 7–11) were obtained by the alkylation of a tertiary diamine with n–iodoalkanes or n–bromoalkanes. The synthesized group of surfactants contain a modified hydrophobic part and a counterion in the form of iodide or bromide (Scheme 1).

Scheme 1.

Synthesis of 3-methylaza-1,5-pentane-bis(N,N-dimethyl-N-alkylammonium) diiodides (compounds 1–6) and dibromides (compounds 7–11).

The other group of gemini surfactants with a secondary nitrogen atom in the spacer (Scheme 2) were prepared by alkylation of N,N,N′,N′-tetramethyl-1,4,7-triazaheptane with iodoalkanes in acetonitrile (compound 19–21). Further alkylation in acetonitrile in the presence of potassium carbonate leads to gemini surfactants with an alkylated nitrogen atom in the spacer (compound 12–18). The details of synthetic procedures of all obtained products are given in the Supplementary Material.

Scheme 2.

Synthesis of 4-aza-1,7-heptane-bis(N,N-dimethyl-N-alkylammonium) diiodides (19–21); 4-alkylaza-1,7-heptane-bis(N,N-dimethyl-N-alkylammonium) dibromides (12–16) and diiodides (17–18).

The structure of all synthesized compounds was determined by ¹H and ¹³C NMR, ESI-MS spectra, or elemental analysis. Moreover, 2D COSY (Correlation Spectroscopy), HMBC (Heteronuclear Multiple Bond Correlation), and HSQC (Heteronuclear Single Quantum Correlation) spectra were performed for compound (1) and (8).

The ¹H NMR spectra of compounds (1–21) show singlets in the range 3.43–3.19 ppm assigned to the N⁺–(CH₃)₂ protons. The characteristic singlet of N–CH₃ of compound (1–11) were observed in the range 2.55–2.53 ppm. Proton singlets at 0.88–0.86 ppm were assigned to CH₃(h). The other multiplets ranging from 1.76–1.25 ppm were assigned to methylene groups of the alkyl chain. These assignments are confirmed by ¹H–¹H COSY, which gives the relationship between protons in the molecule. The ¹H–¹H COSY spectra of compound (8) confirm the structure of the compound (Figure 1).

Figure 1.

Spin–spin couplings obtained from ¹H–¹H COSY spectra of (a) compound (1) and (b) compound (8).

The ¹H–¹³C HMBC provides additional information on the structure of compound (8) (Figure 2). Protons (a) correlated with the carbon atoms (b,c), but they did not correlate with the carbon atoms (f,g,h). It confirms that the alkyl chains are linked to the side nitrogen atoms in the synthesized molecule.

Figure 2.

The ¹H–¹³C HMBC spectra of compound (8).

Chemical shifts of the carbon atoms indicated the occurrence of a relationship between the length of the alkyl chain and the chemical shifts from the carbon atoms located in the vicinity to the quaternary nitrogen atom. Two important signals for the N⁺–CH₃ group and N–CH₃ were present at 51.57–51.52 ppm and 43.15–43.05 ppm, respectively. The spectra show characteristic signals at 14.01–13.94 ppm, which are assigned to CH₃. The details of the structural analysis of all obtained products are given in the Supplementary Material.

2.2. MIC Study

Minimal inhibitory concentrations (MIC), i.e., the minimum concentration of the microbiocide at which further growth of tested microorganisms is stopped, have been determined for all azapolymethylene gemini surfactants against bacteria: E. coli, P. aeruginosa, S. aureus, and B. subtilis as well as microscopic fungi: C. albicans, A. niger, and P. chrysogenum.

The MIC values of 3-methylaza-1,5-pentane-bis(N,N-dimethyl-N-alkylammonium) diiodides (1–6) were shown in Table 1 and of 3-methylaza-1,5-pentane-bis(N,N-dimethyl-N-alkylammonium) dibromides (7–11) were shown in Table 2.

Table 1.

Minimal inhibitory concentrations against fungi of 3-methylaza-1,5-pentane-bis-(N,N-dimethyl-N-alkylammonium) diiodides.

Compound	Minimal Inhibitory Concentration (mM)
	C. albicans	A. niger	P. chrysogenum
1	0.960	1.914	0.475
2	0.110	0.210	0.110
3	0.041	0.082	0.041
4	0.037	0.076	0.076
5	0.071	0.142	0.071
6	0.268	0.134	0.134

Table 2.

Minimal inhibitory concentrations against fungi and bacteria of 3-methylaza-1,5-pentane-bis-(N,N-dimethyl-N-alkylammonium) dibromides.

Compound	Minimal Inhibitory Concentration (mM)
	C. albicans	A. niger	E. coli	P. aeruginosa	S. aureus
7	0.126	0.500	0.126	0.008	0.016
8	0.023	0.116	0.007	0.003	0.003
9	0.054	0.054	0.002	0.013	0.027
10	0.049	0.390	0.006	0.099	0.204
11	0.092	0.739	0.092	0.369	0.369

The highest MIC values against tested fungi were obtained for the derivatives with the shortest alkyl chains. The relationships between MIC and the number of carbon atoms in the alkyl substituent are of parabolic type (Figure 3), with the minimum around C12–C14; the highest antimicrobial activity was observed for the dodecyl and tetradecyl derivatives. It concerns also A. niger, which is usually more resistant to microbiocides than other microscopic fungi and it is very difficult to eradicate from any medical or food industry environment.

Figure 3.

The relationships between MIC and the number of carbon atoms in the alkyl substituent for 3-methylaza-1,5-pentane-bis(N,N-dimethyl-N-alkylammonium) dibromides (7–11).

MIC values of 3-methylaza-1,5-pentane-bis(N,N-dimethyl-N-alkylammonium) dibromides against bacteria: E. coli, P. aeruginosa, and S. aureus were lower than those for microscopic fungi. Similarly, to the effects observed previously, the MIC values against bacteria at first decreased with the increasing length of the hydrocarbon chain, reached the lowest value for the dodecyl and tetradecyl substituent, and then increased again. The antimicrobial activity of dimeric alkylammonium salts is dependent on the length of the hydrocarbon chain. The shorter hydrocarbon chain is not able to penetrate the cell wall; an excessively long substituent is in turn too flexible and also cannot infiltrate the cell wall [30].

It is worth noting, that the antimicrobial activity of gemini surfactants depends not only on the length of the hydrocarbon chains or spacer but also on the type of counterion. In our research, it can be concluded that the diiodide MICs are generally lower than that of dibromides. However, around the smallest MIC for dodecyl and tetradecyl substituents, these values were very similar (Figure 4), which means that decontamination of any area can be done using optimized concentrations of 3-methylaza-1,5-pentane-bis(N,N-dimethyl-N-alkylammonium) dibromides and diiodides without the need to use a high concentration of microbiocides to effectively remove A. niger.

Figure 4.

The relationship between MIC against A. niger and the number of carbon atoms in substituent for 3-methylaza-1,5-pentane-bis(N,N-dimethyl-N-alkylammonium) diiodides (2–6) and dibromides (7–11).

The MIC values against fungi and bacteria for 4-aza-1,7-heptane-bis(N,N-dimethyl-N-alkylammonium) diiodides (19–21) are placed in Table 3. Comparing MIC values against fungi of 3-methylaza-1,5-pentane-bis-(N,N-dimethyl-N-alkylammonium) diiodides (compounds 3,5,6) and 4-aza-1,7-heptane-bis(N,N-dimethyl-N-alkylammonium) diiodides (compounds 19–21) it was seen that gemini surfactants with a secondary amino group in the spacer have better antifungal activity. The lower MIC values against bacteria in this group of gemini surfactants show compounds with dodecyl substituents in the hydrophobic part.

Table 3.

Minimal inhibitory concentrations against fungi and bacteria of 4-aza-1,7-heptane-bis(N,N-dimethyl-N-alkylammonium) diiodides.

Compound		Minimal Inhibitory Concentration (mM)
	C. albicans	A. niger	P. chrysogenum	E. coli	P. aeruginosa	S. aureus	B. subtilis
19	0.010	0.0403	0.0201	0.0050	0.0050	0.0006	0.0006
20	0.0353	0.0701	0.0701	0.0701	0.0701	0.0087	0.0085
21	0.0165	0.0334	0.0165	0.0342	0.0659	0.0165	0.0165

Gemini surfactants based on azapolymethylene spacers showed slightly lower MIC values in comparison to analogous gemini surfactants with polymethylene spacers. Classic gemini surfactant; hexamethylene-1,6-bis(N,N-dimethyl-N-dodecylammonium) dibromide shows MIC (mM): 0.12, 0.12, and 0.06 against C. albicans, A. niger, P. chrysogenum, and 0.087, 0.0073, and 0.0036 against E. coli, P. aeruginosa and S. aureus, respectively [36,37,38].

The MIC values against microorganisms of gemini surfactants with alkylaza-polymethylene spacers are shown in Table 4. Antifungal activity in this group of compounds grew with the increasing length of the middle chain, but differences between MIC values were not too large. The highest antibacterial activity was observed for compounds with the dodecyl substituent in the middle amine group for bromides. Antimicrobial activity of tested 4-alkylaza-1,7-heptane-bis(N,N-dimethyl-N-alkylammonium) diiodides is higher than for tested dibromide. This is probably due to the fact that dibromides have octadecyl chains at quaternary nitrogen atoms.

Table 4.

Minimal inhibitory concentrations against fungi and bacteria of 4-alkylaza-1,7-heptane-bis(N,N-dimethyl-N-alkylammonium) dibromides (12–16) and diiodides (17–18).

Compound		Minimal Inhibitory Concentration (mM)
	C. albicans	A. niger	P. chrysogenum	E. coli	P. aeruginosa	S. aureus	B. subtilis
12	0.0647	0.1787	0.0647	0.0647	0.0647	0.0162	0.0647
13	0.0317	0.1257	0.0628	0.0317	0.0628	0.0157	0.0628
14	0.0308	0.1222	0.0611	0.0153	0.0308	0.0076	0.0308
15	0.0299	0.1189	0.0594	0.0299	0.0299	0.0299	0.0594
16	0.0292	0.0579	0.0292	0.0292	0.0292	0.0292	0.0292
17	0.0082	0.0332	0.0165	0.0332	0.0332	0.0041	0.0082
18	0.0141	0.0559	0.0282	0.0559	0.0559	0.0282	0.0282

The comparison of 4-dodecylaza-1,7-heptane-bis(N,N-dimethyl-N-dodecylmmonium) diiodide (17) and 4-aza-1,7-heptane-bis(N,N-dimethyl-N-dodecylmmonium) diiodide (19) showed that lower MICs against all tested bacteria have got compounds with a secondary amine group in the spacer (Figure 5). On the other hand, the antifungal activity of 17 was lower than for 19, except MIC against C. albicans. This is consistent with the fact that more hydrophobic compounds have a higher antifungal activity.

Figure 5.

Comparison of two compounds, (17) and (19), having different hydrophobicity and their MIC (mM).

2.3. CMC Determination

The CMC was determined using a conductometric titration [39,40]. The ratio of the slopes above and below the CMC provided an estimation of the counterion binding parameter, β. The standard Gibbs energy of micellization (ΔG°_mic) provides information that micelles formation is spontaneous and can be calculated using [14]:

$${\mathsf{\Delta }\mathrm{G}°}_{mic}=\mathrm{RT}\left(\frac{1}{2}+\mathsf{\beta }\right)\mathrm{lncmc}–RT\ln 2$$ (1)

where β is ionization degree, the CMC is expressed in mol/dm³, T is the temperature in Kelvin (K), and R is the gas constant.

Parameters describing micelles formation obtained from conductivity measurement for 3-methylaza-1,5-pentane-bis-(N,N-dimethyl-N-alkylammonium) salts and 4-aza-1,7-heptane-bis (N,N-dimethyl-N-alkylammonium) diiodides are presented in Table 5 and Table 6, respectively.

Table 5.

Critical micelle concentration (CMC), counterion binding parameter (β) and Gibbs free energy of micellization (ΔG°_mic) of 3-methylaza-1,5-pentane-bis-(N,N-dimethyl-N-alkyl-ammonium) diiodides (1–6) and dibromides (7–11) at 25 °C.

Compound Number	CMC (mM)	β	ΔG°mic (kJ/mol)
1	3.46 ± 0.11	0.44	−14.91
2	2.46 ± 0.12	0.45	−21.81
3	0.75 ± 0.06	0.52	−18.12
4	0.35 ± 0.02	0.51	−19.66
5	0.11 ± 0.01	0.43	−22.72
6	0.06 ± 0.01	0.43	−24.10
7	2.78 ± 0.13	0.38	−14.55
8	1.06 ± 0.09	0.53	−38.20
9	0.48 ± 0.07	0.51	−20.27
10	0.15 ± 0.02	0.37	−24.28
11	0.08 ± 0.01	0.29	−40.20

Table 6.

Critical micelle concentration (CMC), counterion binding parameter (β), and Gibbs free energy of micellization (ΔG°_mic) of 4-aza-1,7-heptane-bis(N,N-dimethyl-N-alkylammonium) diiodides at 25 °C.

Compound Number	CMC (mM)	Β	ΔG°mic (kJ/mol)
19	1.001 ± 0.121	0.84	−22.53
20	0.321 ± 0.050	0.49	−22.30
21	0.021 ± 0.003	0.81	−39.05

CMC of gemini surfactants depends on many factors such as alkyl chain length, number of quaternary nitrogen atoms, counterion, functionalization of the spacer, and hydrophobic chain [15,30,40,41]. CMC decreases as the length of the hydrocarbon chain increases. For example, an increase in the length of the hydrocarbon chain from 8 to 18 carbon atoms causes a decrease in CMC of 3.46 to 0.06 mM (compounds (1–6)). This is in agreement with data presented previously with regard to the relationship between gemini surfactants with the polymethylene linker and spacer functionalized with an ether group [42]. Comparing the effect of the counterion, it can be stated that bromides have lower CMC than analogous iodides.

The values of the counterion binding parameter (β) provide information about an average number of counterions per surfactant ion in the micelle [43,44]. For compounds in the same homologous series, β increases with the elongation of the alkyl chain, reaching the highest value for dodecyl derivative, and then decreases with the elongation of the alkyl chain. This correlation is characteristic of gemini surfactants [42]. The big value of β is caused by the stronger binding of the counterion to the micelles, suggesting closer packing of the hydrophilic parts of surfactant and higher surface charge density at the micelle solution interface [42]. The standard Gibbs energy values of the micellization process (ΔG°mic) for gemini surfactants were negative. This means that the micellization process was spontaneous. In addition, ΔG°mic decreases with the elongation of the alkyl chain indicating that the aggregation process is driven by the hydrophobic part of surfactants.

In a group of 4-aza-1,7-heptane-bis(N,N-dimethyl-N-alkylammonium) diiodides dependencies CMC was similar to those described earlier; CMC decreased as the length of the hydrocarbon chain increased.

In the case of 4-alkylaza-1,7-heptane-bis(N,N-dimethyl-N-alkyl-ammonium) salts, another long hydrophobic chain was introduced to the molecule, significantly affecting CMC of gemini surfactants (Table 7).

Table 7.

Critical micelle concentration (CMC), counterion binding parameter (β), and Gibbs free energy of micellization (ΔG°_mic) of 4-alkylaza-1,7-heptane-bis(N,N-dimethyl-N-alkylammonium) dibromides (12–16) and diiodides (17,18) at 25 °C.

Compound Number	CMC (mM)	β	ΔG°mic (kJ/mol)
12	0.063 ± 0.003	0.65	−30.80
13	0.032 ± 0.002	0.40	−26.19
14	0.020 ± 0.001	0.37	−26.30
15	0.013 ± 0.002	0.37	−27.31
16	0.006 ± 0.001	0.31	−26.54
17	0.064 ± 0.003	0.43	−25.17
18	0.033 ± 0.004	0.42	−26.51

Based on the data presented, it can be noted that the introduction of additional long hydrocarbon chains to the molecule, causes increases in surface activity and decreases in CMC values. The relationship between log CMC and the number of carbon atoms in a middle hydrophilic chain decreased linearly (Figure 6), analogous to conventional gemini surfactants [42].

Figure 6.

Variation in log CMC with the number of carbon atoms in the middle hydrocarbon chain for 4-alkylaza-1,7-heptane-bis (N,N-dimethyl-N-alkylammonium) dibromides (12–16).

The counterion binding parameter for gemini surfactants with additional alkyl chains decreases with the elongation of the central alkyl chain. When β is the smallest, the counterion is more strongly bound by aggregates, creating more compacted micelles because of the increasing shielding of the electrostatic charges of the counterion and the cationic part of the surfactant. Gibbs free energy of micellization of this group of gemini surfactants was negative, which clearly shows that the micellization process is spontaneous. The longer the middle alkyl chain, the greater the tendency to form micelles.

There was no simple correlation between MIC and CMC values [45], but for compounds with short or medium chains (1–4, 7–9, 19), the MICs against all tested microorganisms were lower than CMC. In other cases, for gemini with hexadecyl or octadecyl hydrocarbon chains (5, 6, 10, 11–18, 20, 21) MICs were comparable or higher than CMC, due to their lower biocidal activity than gemini with the dodecyl substituent.

3. Materials and Methods

3.1. Materials Used

N,N,N′,N′,N″-pentamethyl-1,4,7-triazaheptane, 3,3′-iminobis(N,N-dimethylpropylamine), all n-akylbromides and n-alkyliodides were purchased from Sigma-Aldrich (Poznań, Poland). Purity of reagents was at least 95%. Acetonitrile was purchased from VWR chemicals; purity for HPLC, and potassium carbonate was bought from P.P.H. Stanlab (Lublin, Poland). All reagents and solvents were obtained without further purification.

3.2. Experimental Methods

3.2.1. Spectroscopic and Spectrometry Characterization of Gemini Surfactants

The NMR spectra were measured with a Varian model VNMR-S 400 MHz (Oxford, UK) operating at 403 and 101 MHz for ¹H and ¹³C, respectively. The ¹³C and ¹H chemical shifts were measured in CDCl₃ and TFA-d relative to an internal standard of TMS. All proton and carbon-13 resonances were assigned by ¹H(COSY) and ¹³C (HETCOR). All 2D NMR spectra were recorded at 298 K on a Bruker Avance DRX 600 spectrometer (Billerica, MA, USA) operating at the frequencies 600.315 MHz (1H) and 150.963 MHz (13C) and equipped with a 5 mm triple-resonance inverse probe head (1H/31P/BB) with a self-shielded z gradient coil (90° 1H pulse width 9.0 μs and 13 C pulse width 13.3 μs).

The ESI (electron spray ionization) mass spectra and elemental analysis were recorded on a Waters/Micromass (Manchester, UK) ZQ mass spectrometer equipped with a Harvard Apparatus (Saint Laurent, Canada) syringe pump. The sample solutions were prepared in methanol at a concentration of approximately 10⁻⁵ M. The standard ESI-MS mass spectra were recorded at the cone voltage 90 V.

3.2.2. Elemental Analysis

The measurements were carried out on a FLASH 2000 elemental analyzer (Delft, Netherlands) with a thermal conductivity detector.

3.2.3. Conductivity Measurement

Conductivity measurements were carried out using a (CO 300, VWR, Gdańsk, Poland) Conductivity Meter with two-pole measuring cell (Ø 12 mm, stainless steel). The instrument was calibrated using a standard (147 µS/cm at 25 °C). Deionized double distilled water was used in all the experimental work, and its specific conductivity value was around 1–2 × 10⁻⁶ S/cm.

3.2.4. MIC Determination

Gemini compounds were tested for antimicrobial activity against bacteria: Escherichia coli ATCC 10536, Pseudomonas aeruginosa ATCC 85327, Staphylococcus aureus ATCC 6538, Bacillus subtilis ATCC 6633, and fungi: Candida albicans ATCC 10231, Aspergillus niger ATCC 16401, and Penicillium chrysogenum ATCC 60739. The MIC values for bacteria and yeast were determined by a tube standard two-fold dilution method [36]. The 24-h culture of bacteria in TSB medium and yeast culture in MEB medium were centrifuged (8000 rpm, for 10 min), resuspended in physiological salt solution, and diluted 100-fold (10⁷ cfu/mL bacteria and 10⁶ cfu/mL yeast). One milliliter of microorganism suspension was mixed with 1 mL of media (TSB for bacteria, MEB for yeast) containing serial dilutions of the tested compounds and incubated at 37 °C for 24 h—bacteria, 48 h—yeast. As a growth control, a suspension of microorganisms in TSB/MEB medium without the biocides was used. The MICs were defined as the lowest concentration of the compounds at which there was no visible growth. The test was repeated three times.

4. Conclusions

Derivatives of 3-methylaza-1,5-pentane-bis(N,N-dimethyl-N-alkylammonium) salts, 4-aza-1,7-heptane-bis(N,N-dimethyl-N-alkylammonium) diiodides, and 4-alkyl-aza-1,7-heptane-bis(N,N-dimethyl-N-alkylammonium) salts were obtained in the reaction of alkylamines with n–bromoalkanes or n–iodoalkanes, respectively. The structures of the title compounds were confirmed by ¹H and ¹³C NMR, ESI-MS spectra and elemental analysis, and HMBC and HSQC spectra. We proved that the MIC of gemini surfactants with azapolymethylene spacers reached the minimum value for tetradecyl and dodecyl derivatives. CMC of the synthesized surfactants are very low and strongly depends on the length of the carbon chain; the longer the hydrocarbon chain, the lower the CMC values. Gemini surfactants with additional hydrocarbon chains in the spacer show a lower CMC value, and lower MIC against fungi, while higher MIC values against bacteria in comparison to the derivative with the amine group in the spacer were observed.

Supplementary Materials

The following are available online at https://www.mdpi.com/1420-3049/25/18/4054/s1, Supplementary Materials contain details on the synthesis and analysis of compounds.

Author Contributions

Conceptualization, I.K.; methodology, I.K. and A.S.; software, M.P.; validation, M.P. and A.K.; formal analysis, M.P.; investigation, I.K.; resources, I.K.; data curation, M.P. and A.K.; writing—original draft preparation, I.K. and M.P.; writing—review and editing, A.S.; visualization, A.K.; supervision, I.K.; project administration, I.K.; funding acquisition, I.K. All authors have read and agreed to the published version of the manuscript.

Funding

This work has been supported by the subsidy from the Faculty of Chemistry, Adam Mickiewicz University for maintaining research potential (I.K.).

Conflicts of Interest

The authors declare no conflict of interest.