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. 2020 Oct 5;14:564054. doi: 10.3389/fnbeh.2020.564054

Figure 1.

Figure 1

Corticotropin-releasing factor (CRF) increases dopamine release in the nucleus accumbens (NAc) in stress-naïve males and females. (A) In the stress-naïve experimental group, mice remained in their home cage for the duration of the experiment. (B) Representative dopamine release evoked by electrical stimulation (dashed line) before and after application of 100 nM CRF to a NAc brain slice collected from a stress-naïve male (mean ± SEM for five consecutive stimulations, top) and corresponding two-dimensional plots depicting changes in faradaic current (pseudocolor) with time as the abscissa and applied potential as the ordinate (bottom). (C) Baseline-normalized mean peak amplitude of electrically evoked dopamine release over time in response to vehicle (n = 9) or 100 nM CRF (n = 11) application to NAc slices from stress-naïve males. (D) Baseline-normalized mean peak amplitude of dopamine release 20–30 min after vehicle (n = 9) or 100 nM CRF (n = 11) application to NAc slices from stress-naïve males. (E) Representative dopamine release evoked by electrical stimulation (dashed line) before and after application of 100 nM CRF to a NAc brain slice from a stress-naïve female (mean ± SEM for five consecutive stimulations, top) and corresponding color plots (bottom). (F) Baseline-normalized mean peak amplitude of electrically evoked dopamine release over time in response to vehicle (n = 10), 100 nM CRF (n = 8), or 1 μM CRF (n = 8) application to NAc slices from stress-naïve females. (G) Baseline-normalized mean peak amplitude of dopamine release 20–30 min after vehicle (n = 10), 100 nM CRF (n = 8), or 1 μM CRF (n = 8) application to NAc slices from stress-naïve females. Error bars, SEM. *P < 0.05, **P < 0.01, ***P < 0.001 vs. vehicle.