Table 4.

qPCR Primer Sequences and Conditions

Gene	Primer (5′ to 3′)	Annealing Temp (°C)	Amplicon Size (bp)
Rho	Fwd: TGAGGATAGCACCCATGCAA	62	397
	Rev: GGCGCAGCTTCTTGAATCTC
Gnat1	Fwd: CCCACTGAGCAGGACGTGTT	62	176
	Rev: AGCGCAGCGATGAAAATGAT
Pde6β	Fwd: TCCTCTGGTCAGCCAATAAGGT	62	200
	Rev: TGTTCGTGGGCCTGAGTATG
Opnsw	Fwd: GAGGCCTTCTTGGGCTCTGTA	62	200
	Rev: ATGAACCTGCTCCAGCCAAA
Gnat2	Fwd: CCAGCTGGACCGGATTACAG	62	189
	Rev: CAGGTGACTCCCTCGAAGCA
Gnb3	Fwd: GGGACATCTGGCCAAGATCTAT	62	72
	Rev: TCCTGCGAGGCACTTACGA
Eef2	Fwd: TGTCAGTCATCGCCCATGTG	62	123
	Rev: CATCCTTGCGAGTGTCAGTGA