Table 5. Enzymatic sensitivity and heat stability profile of antimicrobial activities.
These 19 isolates showed antagonistic activities against Clostridium perfringens.
| Isolate | Activity* | Proteinase K (proK) | Pronase E (proE) | Trypsin | Lipase | Sensitive to enzymes | Resistant to (t °C) |
|---|---|---|---|---|---|---|---|
| #01 | 13.67 ± 0.58 | 10.67 ± 0.58 | 10.67 ± 0.58 | 14.00 | 13.67 ± 0.58 | proE, proK | 60 |
| #02 | 11.00 | 9.00 | – | 10.67 ± 0.58 | 11.00 | proE, proK | 60 |
| #04 | 9.00 | – | – | – | 9.00 | proE, proK, trypsin | – |
| #05 | 23.67 ± 0.58 | 23.67 ± 0.58 | 23.67 ± 0.58 | 23.67 ± 0.58 | 23.67 ± 0.58 | – | 60 |
| #06 | 14.67 ± 0.58 | 13.00 ± 1.00 | 10.33 ± 0.58 | – | – | proE, trypsin, lipase | – |
| #08 | 13.67 ± 0.58 | 12.00 | 11.33 ± 0.33 | 13.33 ± 0.33 | 14.00 | proE, proK | 60 |
| #10 | 9.00 | – | – | – | 9.00 | proE, proK, trypsin | – |
| #11 | 17.00 | 15.00 | – | 15 ± 0.58 | – | proE, proK, lipase | 60 |
| #12 | 11.67 ± 0.58 | 8.67 ± 0.58 | – | – | 12.00 | proE, proK, trypsin | – |
| #13 | 16.00 | 16.00 | 16.00 | 12.57 ± 0.58 | 18.33 ± 0.58 | trypsin | 60 |
| #14 | 8.67 ± 0.58 | 8.67 ± 0.58 | – | – | – | proE, proK, trypsin, lipase | – |
| #15 | 8.67 ± 0.58 | – | – | 9.00 | 9.00 | proE, trypsin | – |
| #16 | 12.00 ± 0.58 | – | – | – | 12.00 | proE, proK, trypsin | – |
| #17 | 12.33 ± 0.58 | 9.67 ± 0.58 | – | – | 8.67 ± 0.58 | proE, proK, trypsin | – |
| #18 | 9.67 ± 0.58 | 10.33 ± 0.58 | 10.00 | 10.33 ± 0.58 | 10.00 | – | – |
| #19 | 13.67 ± 0.58 | 14.00 | 13.67 ± 0.58 | 13.67 ± 0.58 | 12.67 ± 0.58 | – | 60 |
| #20 | 11.00 | 11.00 | 8.67 ± 0.58 | 11.00 | 11.00 | proE | – |
| #21 | 15.00 | 11.00 | 12.33 ± 0.58 | 15.00 | 11.33 ± 0.58 | proE, proK, lipase | – |
| #23 | 13.00 | 12.33 ± 0.58 | – | 12.33 ± 0.58 | 12.67 ± 0.58 | proE | – |
Notes.
*
Value indicated the diameter of halo zone of antimicrobial activity in millimetres including well diameter of 6 mm
‘–’
indicates no zone of clearing.
Underlined enzyme indicated a loss of activity after enzymatic treatment. The values of temperature resistance were recorded after 3 hours of incubation. The numbers in the table represented the mean value (±standard deviation) of killing diameters from the measurement of three replicates. These 19 isolates showed antagonistic activities against Clostridium perfringens.