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. 2020 Oct 5;10:542007. doi: 10.3389/fonc.2020.542007

Figure 1.

Figure 1

Elevated 14-3-3ζ expression positively correlates with poor prognosis of lung adenocarcinoma (LUAD) patients. (A) Relative mRNA levels of 14-3-3ζ in different tumors were analyzed by Gene Expression Profiling Interactive Analysis (GEPIA). *p < 0.05 (B) Representative immunostaining images for 14-3-3ζ expression in 128 human LUAD tissues with epidermal growth factor receptor (EGFR) mutations (tumor) and 31 non-tumoral lung tissues (non-tumor). Original magnification, ×400; Scale bar = 200 μm. Histogram showing percentages of 14-3-3ζ expression in tumor and non-tumor tissues. ***p < 0.001 (C) Western blot analysis of 14-3-3ζ expression in lysates originating from 10 human LUAD samples with EGFR mutant (T) and matched adjacent normal tissues (N). Right panel: quantification of relative expression levels of 14-3-3ζ. β-actin was used as a loading control. *p < 0.05. (D) Kaplan–Meier analysis of overall and disease-free survival (DFS) for high and low 14-3-3ζ expression levels in pancancer determined by GEPIA. (E) Kaplan–Meier analysis of overall and DFS for high and low 14-3-3ζ expression levels in LUAD determined by GEPIA.