Table 2.

Central Composite Design Runs for the 5 Different Factors Tested Showing the Observed and Predicted Responses

Run No	Starch (g/L)	Ammonium Chloride (g/L)	pH	Temperature (°C)	Agitation (rpm)	Observed Inhibition Zone (mm)	Predicted Inhibition Zone (mm)
1	3	3	6	35	225	21	21.9
2	1	5	6	40	300	17	17.9
3	1	5	8	40	150	12	13.8
4	1	1	8	30	150	0	0.8
5	3	3	7	30	225	26	25.1
6	5	1	6	30	150	22	24
7	5	3	7	35	225	33	33
8	3	3	7	35	225	28	28
9	3	3	8	35	225	15	17.6
10	3	3	7	35	225	28	28
11	5	5	6	30	300	30	30.7
12	3	3	7	40	225	19	23.5
13	3	3	7	35	225	28	28
14	1	5	6	30	150	19	21
15	1	1	8	40	300	14	14.4
16	5	5	6	40	150	23	22.9
17	1	5	8	30	300	11	11
18	3	3	7	35	225	28	28
19	5	1	6	40	300	17	20.5
20	3	3	7	35	225	28	28.1
21	1	1	6	40	150	13	14.9
22	3	5	7	35	225	31	33.1
23	5	5	8	30	150	27	28.1
24	3	3	7	35	150	25	27.1
25	5	1	8	40	150	16	16.3
26	1	3	7	35	225	18	23.1
27	5	5	8	40	300	20	22.5
28	3	1	7	35	225	27	28.5
29	1	1	6	30	300	15	15.1
30	3	3	7	35	300	26	29
31	3	3	7	35	225	28	28.1
32	5	1	8	30	300	24	25.1