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. 2020 Oct 19;15(10):e0235103. doi: 10.1371/journal.pone.0235103

Fig 4. Elevated levels of PCNA sensitizes mec1-1 mutant cells to MMS.

Fig 4

[A] Elevated levels of PCNA does not impact tel1Δ1 and mec1-1 mutant cells under high temperature stress. 10-fold serial dilution of indicated yeast strains plated on selective medium plates and incubated at 23°C, 34°C, and 37°C for 2 days. [B] Elevated levels of PCNA specifically sensitizes mec1-1 mutant cells when exposed to DNA damage. 10-fold serial dilution of indicated yeast strains plated on selective medium plates containing no MMS or 0.01% MMS and incubated at 23°C for 2 days. [C] Rad53 checkpoint is not activated in mcd1-1 elg1Δ double mutant cells. DNA content are shown for cells in log phase, synchronized in G1 with alpha factor, and released and shifted to 37°C and arrested in pre-anaphase. Wildtype cells exposed to 0.05% MMS was used as a positive control for Rad53 activation. Rad53 was detected using a Rad53 specific antibody, and PGK was used as a loading control.