TABLE 3.

Differential Expression of Activated Platelet-Specific Spliced RNA Transcripts in CFP of Isolated Severe fTBI vs. t-Control Patients

Gene Name	Gene Name/Function	fTBI/t-Control (Fold Change)	Platelet Gene Rank
AKT3	RAC-γ serine/threonine protein kinase	↓ >4×	2,581
ANKRD12	Ankyrin-repeat containing transcriptional regulator	↓ > 10×	968
ARHGAP18	Rho GTPase activating protein	↓ > 5×	580
ARHGAP21	CDC42 GTPase activating protein, actin regulation	↓ >8×	1,603
ARHGAP21	CDC42 GTPase activating protein, actin regulation	↓ > 1,000×	1,603
ARHGEF12	Rho guanine nucleotide exchange factor	↓ >2×	1,271
ARHGEF12	Rho guanine nucleotide exchange factor	↑3×	1,271
CALD1	Caldesmon 1, cytoskeletal regulation	↑30×	1,627
CCL5	Histamine and eosinophil activation	↓ >8×	72
CDK2AP1	Cyclin-dependent kinase 2-associated protein 1	↓ > 25×	451
CHD9	Chromodomain helicase DNA binding protein 9	↓ >8×	2,251
CSNK1G3	Casein kinase1 γ 3, intracellular signaling	↓ >8×	1,850
DNM3	Microtubules and vesicular transport	↑14×	602
DNM3	Microtubules and vesicular transport	↑2×	602
EIF4G3	Eukaryotic translation initiation factor 4 γ 3	↓ > 250×	877
F13A1	Factor XIII A Subunit	↓ > 25×	47
FIP1L1	Factor interacting with PAPOLA and CPSF1, pre-mRNA 3′ end processing	↓ > 207×	1,668
FNBP1L	Formin binding protein 1-like, cytoskeletal regulation	↓ > 150×	4,067
GRB14	Growth factor receptor bound protein 14	↓ > 336×	3,729
HBA1	Hemoglobin subunit α 1	↓ > 348×	25
IQGAP2	IQ motif containing GTPase activating protein, cytoskeletal regulator	↓ > 22×	1,720
IQGAP2	IQ motif containing GTPase activating protein, cytoskeletal regulator	1×	1,720
IQGAP2	IQ motif containing GTPase activating protein, cytoskeletal regulator	↓ > 17×	1,720
KIF2A	Kinesin family member, microtubule motor	↑6×	164
LIMS1	LIM Zn finger domain containing Integrin signaling adaptor	1×	209
LTBP1	Latent transforming growth factor binding protein 1	↓ > 46×	824
MAP4K5	MAP kinase kinase kinase kinase 5, JNK signaling	↓ > 6×	5,254
MBNL3	Muscleblind-like splicing regulator 3	↓ > 176×	1,394
MLLT3	Super elongation complex subunit	↓ > 51×	4,178
NAP1L1	Nucleosome assembly protein 1-like 1	↑19×	168
NAP1L1	Nucleosome assembly protein 1-like 1	1×	168
NLK	Nemo-like kinase	↓ > 648 ×	2,165
PF4	Chemokine, released from alpha granules	↑3×	13
PF4	Chemokine, released from alpha granules	↑1.5×	13
PLEK	Pleckstrin, platelet activation, and vesicle secretion	↑4×	431
PTGS1	Cyclooxygenase 1, prostaglandin biosynthesis	↓ > 357×	237
PTGS1	Cyclooxygenase 1, prostaglandin biosynthesis	↓ > 406×	237
RAP1B	Regulation of integrin-mediated cell signaling, and outside in signaling in platelets	↓ > 117×	261
RBPMS2	RNA-binding protein with multiple splicing 2	↓ > 118×	8,079
SH3BGRL2	SH3 domain binding glutamate-rich protein-like 2	↓ > 10×	189
SPARC	Osteonectin, activation-dependent platelet secretion, bone, and ECM metabolism	↑1.5×	85
TMSB4X	Thymosin β 4, actin regulation, tissue regeneration	↓ > 1000×	24

Platelet Gene Rank, absolute gene count from normalized ex vivo platelet control²²; GTP, guanosine triphosphate; CDC42, Cell Division Cycle 42; PAPOLA, polynucleotide adenylyltransferase alpha; CPSF1, cleavage and polyadenylation specific factor 1; IQ, isoleucine-glutamine; LIM, Lin11, Isl-1 and Mec-3; MAP, mitogen-activated protein; JNK, c-Jun N-terminal kinase; ECM, extracellular matrix; SH3, Src (Sarcoma) homology 3.