Skip to main content
. 2020 Oct 16;99(42):e22438. doi: 10.1097/MD.0000000000022438

Figure 1.

Figure 1

Mcl-1 protein and mRNA expression decreased in RAW264.7 cells infected with different virulent MTB when treated with Mcl-1 expression signaling pathway inhibitors. RAW264.7 cells were infected with BCG, H37Ra, H37Rv and XJ-MTB at MOI = 10 (bacterium: cell) for 24 hours, then specific signaling pathway inhibitors AG490 (Fig. 1A), PD98059 (Fig. 1B), LY294002 (Fig. 1C) were used to affect Mcl-1 expression signaling pathways. Cells lysate and total RNA was collected, Mcl-1 protein and mRNA expression were detected using western blotting and RT-PCR. The grouping of blots cropped from different gels. Values represent the mean ± SD from three independent experiments. #P < 0.05 VS uninfected control group, ##P < 0.01 VS uninfected control group; ∗P < .05 VS untreated group, ∗∗P < .01 VS untreated group.