Figure 5.

Reduced myogenesis in shPAB is affected by cytoskeletal stabilization and organization. A Representative images of fused control and shPAB cultures. MyHC staining is depicted in green and the nuclei in blue. B Bar chart shows fusion index, which was calculated by the fraction of nuclei within MyHC objects. The average number of nuclei per replicate is denoted in or above each bar. Average and standard deviation are from four replicates. Scale bar is 100 µm. C Cell fusion in the actin-GFP expressing cell lines. Representative images of cell cultures fused for four days, parental or actin-GFP overexpressed control or shPAB cell lines. MyHC is depicted in green and actin-GFP in gray, the merged image shows an overlay between MyHC and actin-GFP. D Bar chart shows fusion index in parental and actin-GFP control and shPAB cultures. Average and standard deviation are from three replicates. The average number of nuclei per replicate is denoted in each bar. Scale bar is 100 µm. E Images show MURC localization (depicted in red) in the actin-GFP (depicted in cyan) fused cell cultures. Scale bars is 35 µm. Statistical significance was assessed using a student’s T-test, * denotes p < 0.05. Every experimental setup was repeated three times.