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. 2020 Oct 19;10:17686. doi: 10.1038/s41598-020-74574-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Hypothetical targets positioning of BeTS-dm and CiTS-dm along A. flavus secondary metabolism. Being ineffective on sclerotia biogenesis while highly inhibitory on AF accumulation, BeTS is suggested to intervene down-stream the bifurcation of secondary metabolism that divide AFs biosynthetic pathway from sclerotia developmental biogenesis. On the contrary, due to the containment effect of CiTS-dm on sclerotia and AF, its target could be expected enough up-stream the bifurcation to interfere with their production, or immediately after, in correspondence of a metabolic knot shared by (and controlling) both processes in A. flavus. Scheme created with Adobe Illustrator and Adobe Photoshop CS6.0 (Adobe Inc., San Jose, CA, USA).