Table 3.
Targeted mass spectroscopy quantified significantly altered fasting plasma metabolites with P and q values < 0.05 in obese controls (n = 40) and obese + T2DM patients (n = 40).
| Metabolite class | Metabolite | Control mean conc | T2DM mean conc | P-value | q value (BH adjusted) | Effect size | Power (%) |
|---|---|---|---|---|---|---|---|
| Sugars | H1 | 4989.75 | 7399.65 | 1.40E−09 | 2.00E−07 | 1.336 | 100.0 |
| Phosphatidylcholines | PC.ae.C34.2 | 8.1888 | 5.8055 | 2.75E−08 | 1.97E−06 | 1.389 | 100.0 |
| PC.ae.C36.3 | 5.7717 | 4.2237 | 2.90E−07 | 1.38E−05 | 1.246 | 99.9 | |
| PC.ae.C36.4 | 14.6275 | 11.5653 | 3.97E−07 | 1.42E−05 | 1.207 | 99.9 | |
| PC.aa.C36.2 | 171.5 | 138.66 | 2.19E−06 | 5.23E−05 | 1.065 | 99.9 | |
| PC.aa.C32.3 | 0.4256 | 0.3185 | 3.34E−06 | 6.81E−05 | 1.043 | 99.9 | |
| PC.ae.C38.5 | 11.0617 | 9.3667 | 7.15E−05 | 0.0009 | 0.867 | 99.0 | |
| PC.aa.C34.2 | 272.85 | 234.9 | 8.85E−05 | 0.0011 | 0.863 | 99.0 | |
| PC.ae.C34.3 | 5.8365 | 4.4625 | 0.0002 | 0.0018 | 0.834 | 98.6 | |
| PC.aa.C36.3 | 107.5975 | 91.1925 | 0.0002 | 0.0019 | 0.840 | 98.6 | |
| PC.aa.C38.3 | 38.03 | 31.3475 | 0.0005 | 0.0040 | 0.778 | 97.1 | |
| Lysophosphatidylcholines | lysoPC.a.C18.0 | 20.8625 | 17.3475 | 0.0007 | 0.0055 | 0.810 | 98.0 |
| lysoPC.a.C18.2 | 16.5508 | 12.7825 | 0.0021 | 0.0111 | 0.749 | 96.0 | |
| lysoPC.a.C18.1 | 0.2607 | 0.2068 | 0.0128 | 0.0446 | 0.631 | 87.8 | |
| Sphingomyelins | SM.C16.1 | 20.225 | 16.67 | 5.42E−05 | 0.0008 | 0.925 | 99.6 |
| SM..OH..C14.1 | 6.873 | 5.5665 | 0.0002 | 0.0021 | 0.875 | 99.1 | |
| SM.C20.2 | 0.491 | 0.3835 | 0.0004 | 0.0037 | 0.763 | 96.6 | |
| SM..OH..C22.2 | 10.9228 | 9.164 | 0.0016 | 0.0091 | 0.735 | 95.4 | |
| SM..OH..C16.1 | 3.368 | 2.882 | 0.0141 | 0.048 | 0.575 | 81.3 | |
| Acylcarnitines | C3 DC..C4.OH | 0.0395 | 0.0607 | 9.28E−07 | 2.66E−05 | 0.988 | 99.8 |
| Amino acids | Gln | 560.5 | 480.175 | 0.0002 | 0.0018 | 0.890 | 99.3 |
| His | 87.66 | 77.7075 | 0.001 | 0.0066 | 0.722 | 94.7 | |
| Ala | 315.775 | 379.6 | 0.001 | 0.0066 | 0.760 | 96.4 | |
| Orn | 62.045 | 50.2025 | 0.0011 | 0.0074 | 0.715 | 94.3 | |
| Trp | 66.92 | 58.235 | 0.0013 | 0.0082 | 0.711 | 94.1 |
All patients were obese male/female UAE nationals. Subjects were stratified into obese non-diabetic and obese T2DM groups. The mean plasma concentration (µM) of metabolites with P and q values < 0.05 and power ≥ 80% was reported. Statistical significance (P) was calculated using Student’s t-test, and the results were adjusted for q value (Benjamini–Hochberg adjustment). Post hoc: difference between independent means analyses was applied to calculate effect size and power.