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. 2020 Oct 19;11:5281. doi: 10.1038/s41467-020-19006-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a, b Relative abundance and expression levels of recovered populations represented by rMAGs over time based on MG depth (a) and MT depth (b) of coverage, respectively, representing mapping percentages of MG [26% ± 3% (s.d.)] and MT [27% ± 3% (s.d.)]. The relative abundance of individual rMAGs is grouped based on genus-level taxonomic assignment with rMAGs of unresolved taxonomy grouped in “Other”. Recovered genera with mean abundance below 2% are summarized as a single group (light gray). c, d Ordination of Bray–Curtis dissimilarity of relative abundances, MG (c) and MT (d), of individual rMAGs constrained by selected abiotic factors (metabolite levels, metabolite-ratios, and physico-chemical parameters shown as black arrows with arrow lengths indicating environmental scores as predictors for each factor). Points are colored by month of sampling and point-shape reflects the year of sampling. Thin black lines connecting the points visualize the time course of sampling. Source data are provided as a Source Data file.