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. Author manuscript; available in PMC: 2020 Oct 20.
Published in final edited form as: Curr Pharm Des. 2020;26(34):4330–4337. doi: 10.2174/1381612826666200415171622

Figure 1. T-maze spontaneous alternation in Adora2b−/− mice.

Figure 1.

Wildtype mice (C57BL6/J) were injected with midazolam (10mg/kg i.p.) and compared to saline-treated controls. (A) Experimental setup overview. (B) Adora2b transcript levels in the hippocampus. Brain hippocampus tissue was harvested, mRNA was isolated using RNeasy Mini Kit (Qiagen), cDNA was generated using miScript RT II kits (Qiagen), and Adora2b transcript levels were determined by quantitative real-time RT-PCR (iCycler or iCycler IQ; Bio-Rad Laboratories Inc.), n=5, mean±SD; p<0.05. (C) T maze spontaneous alternation test. (D) T-maze alternation in % from Adora2b−/− mice, n=4–10, mean±SD; p<0.05.