Skip to main content
. Author manuscript; available in PMC: 2020 Oct 20.
Published in final edited form as: Curr Pharm Des. 2020;26(34):4330–4337. doi: 10.2174/1381612826666200415171622

Figure 4. Adora2b−/− mice have less hippocampal C-fos levels.

Figure 4.

(A-D) Brain hippocampus tissue was harvested from wildtype (C57BL6/J) or Adora2b−/− mice, mRNA was isolated using RNeasy Mini Kit (Qiagen), cDNA was generated using miScript RT II kits (Qiagen), and Tnf-α. (A), Il-6 (B), Bdnf (C) and C-fos (D) transcript levels were determined by quantitative real-time RT-PCR (iCycler or iCycler IQ; Bio-Rad Laboratories Inc.), n=4–5, p<0.05, all data are presented as mean ± SD.