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. Author manuscript; available in PMC: 2020 Nov 4.
Published in final edited form as: Mucosal Immunol. 2020 May 4;13(6):908–918. doi: 10.1038/s41385-020-0290-x

Figure 2. Single cell transcriptomic map of immune cells from young and aged bladders.

Figure 2.

(A) Clustering analysis and cell type identification of CD45+ cells from young (n= 3209) and aged bladders (n=4682). Cluster numbers are ordered by most abundant cell type from the merged data set. (B) Map of cells originating from young or aged bladders. (C) Expression of Cxcl13 in scRNA-seq macrophage clusters (numbered as in A and colored as in B). (D ) RT-qPCR of sorted F4/80+CD64+Ly6C- macrophages from young and aged mice (n=3–5/group). **p<0.01, Unpaired t-test with Welch’s correction. Lines at mean.